The Mouse SIGA (Secretory Immunoglobulin A) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of SIGA levels in mouse samples such as serum, plasma, and cell culture supernatants. SIGA is a key component of the mucosal immune system and plays a vital role in protecting the body against infections and harmful pathogens.This kit provides reliable and reproducible results, making it an essential tool for researchers studying immune responses, infectious diseases, and mucosal immunity in mice.
With its high sensitivity and specificity, this kit is suitable for a wide range of research applications and can help advance our understanding of the immune system and develop new therapies for various diseases.Don't miss out on the opportunity to enhance your research with the Mouse SIGA CLIA Kit from Assay Genie. Visit the link above to learn more and order your kit today!
Product Name:
Mouse sIgA (Secretory Immunoglobulin A) CLIA Kit
SKU:
MOES00524
Target:
Mouse sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse sIgA. You can calculate the concentration of Mouse sIgA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
86-96
96-108
Average (%)
109
91
102
1:4
Range (%)
85-100
96-112
85-98
Average (%)
91
102
91
1:8
Range (%)
88-103
90-105
97-109
Average (%)
95
96
102
1:16
Range (%)
87-101
95-110
102-117
Average (%)
94
103
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
100-113
105
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.85
298.27
963.84
99.9
276.95
897.67
Standard deviation
9.57
24.76
75.37
9.36
32.04
62.21
C V (%)
10.42
8.3
7.82
9.37
11.57
6.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sIgA in samples. No significant cross-reactivity or interference between Mouse sIgA and analogues was observed.
