SynonymsSIR2L,SIR2-L,SIR2L2,Silent Information Regulator 2 Protein,NAD-Dependent Deacetylase Sirtuin-2,Silent Mating Type Information Regulation 2 Homolog
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
MODL01189
UPC:
Product Type:
ELISA Kit
Sensitivity:
0.61ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
SIR2L,SIR2-L,SIR2L2,Silent Information Regulator 2 Protein,NAD-Dependent Deacetylase Sirtuin-2,Silent Mating Type Information Regulation 2 Homolog
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse SIRT2 ELISA Kit is specifically designed for the precise quantification of SIRT2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.SIRT2, a member of the sirtuin family of proteins, plays a critical role in regulating various cellular processes such as metabolism, cell cycle progression, and stress response. It is implicated in aging, neurodegenerative diseases, and cancer, making it a valuable biomarker for investigating these conditions and exploring therapeutic interventions.
With the Mouse SIRT2 ELISA Kit, researchers can confidently measure SIRT2 expression levels in mouse samples, advancing their understanding of molecular mechanisms and potential treatment options for diseases associated with SIRT2 dysregulation. Trust in the precision and reliability of this ELISA kit for your research needs.
Product Name:
Mouse Sirtuin 2 (SIRT2) ELISA Kit
Product Code:
MODL01189
Size:
96 Assays
Target:
SIRT2
Synonyms:
SIR2L, SIR2-L, SIR2L2, Silent Information Regulator 2 Protein, NAD-Dependent Deacetylase Sirtuin-2, Silent Mating Type Information Regulation 2 Homolog
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
