Mouse SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit
The Mouse SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit is specifically designed for the accurate and sensitive detection of pulmonary surfactant associated protein D levels in mouse samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.Pulmonary surfactant associated protein D plays a critical role in lung function and immune response, making it a valuable biomarker for studying respiratory diseases and conditions such as acute respiratory distress syndrome (ARDS) and pneumonia.
By accurately measuring pulmonary surfactant associated protein D levels, researchers can gain valuable insights into lung health and potential therapeutic targets.
Product Name:
Mouse SPD (Pulmonary Surfactant Associated Protein D) CLIA Kit
SKU:
MOES00511
Target:
Mouse SPD (Pulmonary Surfactant Associated Protein D)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SP-D. You can calculate the concentration of Mouse SP-D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-103
91-106
Average (%)
92
95
99
1:4
Range (%)
85-99
102-118
87-101
Average (%)
91
110
93
1:8
Range (%)
100-114
92-107
100-116
Average (%)
108
99
106
1:16
Range (%)
85-96
93-107
85-99
Average (%)
91
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
98-111
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.35
293.12
725.81
113.33
298.76
782.86
Standard deviation
13.35
28.02
66.19
14.53
33.25
63.26
C V (%)
12.79
9.56
9.12
12.82
11.13
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SP-D concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SP-D in samples. No significant cross-reactivity or interference between Mouse SP-D and analogues was observed.
