SynonymsPGL3,CYB560, SDH3,CYBL,QPs1,Integral membrane protein CII-3,Succinate-ubiquinone oxidoreductase cytochrome B large subunit,Succinate dehydrogenase cytochrome b560
ReactivityMouse
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
MODL01164
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9CZB0
Sensitivity:
0.071ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PGL3,CYB560, SDH3,CYBL,QPs1,Integral membrane protein CII-3,Succinate-ubiquinone oxidoreductase cytochrome B large subunit,Succinate dehydrogenase cytochrome b560
Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit
The Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA kit is a powerful tool for the precise measurement of SDHC levels in mouse tissue lysates and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.SDHC is a key component of the succinate dehydrogenase complex, playing a critical role in the electron transport chain and cellular respiration.
Dysregulation of SDHC has been linked to various metabolic disorders, making it a valuable target for studying metabolic pathways and potential therapeutic interventions.With its reliable performance and user-friendly protocol, the Mouse SDHC ELISA kit is an essential tool for researchers exploring the complex mechanisms of cellular metabolism and seeking new insights into metabolic diseases.
Product Name:
Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit
Product Code:
MODL01164
Size:
96 Assays
Target:
SDHC
Synonyms:
PGL3, CYB560, SDH3, CYBL, QPs1, Integral membrane protein CII-3, Succinate-ubiquinone oxidoreductase cytochrome B large subunit, Succinate dehydrogenase cytochrome b560
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
