The Mouse TBG (Thyroxine Binding Globulin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TBG levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.TBG is a key protein that plays a critical role in the regulation of thyroid hormone levels in the body.
Dysregulation of TBG levels has been associated with thyroid disorders and other conditions, making it an important biomarker for studying these diseases and developing potential treatments.With its advanced technology and precise methodology, the Mouse TBG CLIA Kit from AssayGenie provides researchers with a valuable tool for studying the role of TBG in various diseases and physiological processes.
Product Name:
Mouse TBG (Thyroxine Binding Globulin) CLIA Kit
SKU:
MOES00563
Target:
Mouse TBG (Thyroxine Binding Globulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TBG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TBG. You can calculate the concentration of Mouse TBG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
84-95
95-111
Average (%)
91
90
103
1:4
Range (%)
94-105
94-109
95-110
Average (%)
99
101
101
1:8
Range (%)
94-107
93-108
86-102
Average (%)
99
101
93
1:16
Range (%)
84-97
99-114
92-103
Average (%)
91
107
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-118
108
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.93
494.64
1898.85
201.17
539.35
1805.72
Standard deviation
19.7
38.58
183.24
23.66
56.25
139.94
C V (%)
10.71
7.8
9.65
11.76
10.43
7.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TBG in samples. No significant cross-reactivity or interference between Mouse TBG and analogues was observed.
