The Mouse TCC (C5b-9) Terminal Complement Complex ELISA Kit is a reliable and sensitive assay designed for the accurate detection of C5b-9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring dependable and consistent results for a variety of research applications.The C5b-9 complex is a critical component of the complement system, playing a key role in immune defense and inflammation.
Dysregulation of this complex has been implicated in various diseases, making it a valuable biomarker for studying immune-related disorders and developing potential therapeutic interventions.Overall, the Mouse TCC (C5b-9) Terminal Complement Complex ELISA Kit is a valuable tool for researchers looking to investigate the role of the complement system in health and disease in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sC5b-9. You can calculate the concentration of Mouse sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
85-98
86-99
Average (%)
105
90
91
1:4
Range (%)
86-97
80-90
86-100
Average (%)
92
85
93
1:8
Range (%)
86-98
87-100
84-98
Average (%)
93
92
90
1:16
Range (%)
93-108
84-97
86-98
Average (%)
100
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.72
6.74
1.06
2.75
6.33
Standard deviation
0.06
0.13
0.26
0.07
0.15
0.21
C V (%)
6.19
4.78
3.86
6.6
5.45
3.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse sC5b-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sC5b-9 in samples. No significant cross-reactivity or interference between Mouse sC5b-9 and analogues was observed.
