The Mouse TF (Tissue Factor) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of tissue factor levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Tissue factor, also known as coagulation factor III, is a key initiator of the blood coagulation cascade, playing a crucial role in hemostasis and thrombosis. Dysregulation of tissue factor has been implicated in various pathological conditions, including thrombotic disorders, inflammation, and cancer.
Therefore, this kit serves as a valuable tool for studying the role of tissue factor in disease pathogenesis and potential therapeutic interventions.Overall, the Mouse TF (Tissue Factor) CLIA Kit offers researchers a reliable and efficient way to measure tissue factor levels in mouse samples, providing valuable insights into its biological functions and clinical implications.
Product Name:
Mouse TF (Tissue factor) CLIA Kit
SKU:
MOES00566
Target:
Mouse TF (Tissue factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TF. You can calculate the concentration of Mouse TF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
88-104
96-110
Average (%)
102
95
101
1:4
Range (%)
97-112
94-108
94-106
Average (%)
104
99
99
1:8
Range (%)
85-100
88-104
91-103
Average (%)
91
96
97
1:16
Range (%)
92-106
87-100
93-104
Average (%)
97
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
94-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.98
19.94
79.55
9.22
19.52
86.84
Standard deviation
1.1
2.01
6.35
1.01
2.24
6.16
C V (%)
11.02
10.08
7.98
10.95
11.48
7.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TF in samples. No significant cross-reactivity or interference between Mouse TF and analogues was observed.
