The Mouse TFF3 (Trefoil Factor 3) Intestinal CLIA Kit is specially designed for the precise measurement of TFF3 levels in mouse serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.TFF3 is a vital protein that plays a key role in maintaining gastrointestinal integrity, wound healing, and mucosal protection. Its dysregulation is linked to inflammatory bowel diseases, digestive disorders, and cancer, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
Overall, the Mouse TFF3 Intestinal CLIA Kit is an invaluable tool for researchers in the field of gastroenterology and cancer biology, providing reliable data for advancing our understanding of intestinal health and disease progression.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TFF3. You can calculate the concentration of Mouse TFF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
102-117
93-107
Average (%)
91
108
98
1:4
Range (%)
97-110
97-114
94-107
Average (%)
102
105
99
1:8
Range (%)
86-100
91-102
101-116
Average (%)
92
97
108
1:16
Range (%)
103-118
92-105
99-111
Average (%)
110
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.19
148.29
490.31
52.93
151.17
490.0
Standard deviation
6.56
15.33
31.23
6.39
12.93
49.98
C V (%)
12.82
10.34
6.37
12.07
8.55
10.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TFF3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TFF3 in samples. No significant cross-reactivity or interference between Mouse TFF3 and analogues was observed.
