The Mouse TGF-beta1 (Transforming Growth Factor beta1) CLIA Kit is specifically designed for the quantitative measurement of TGF-beta1 levels in mouse serum, plasma, and tissue homogenates. This CLIA kit offers high sensitivity and accuracy, ensuring reliable and reproducible results for a variety of research applications.TGF-beta1 is a key regulator of cell growth, differentiation, and immune response, and aberrant TGF-beta1 signaling has been implicated in numerous diseases, including cancer, fibrosis, and autoimmune disorders.
By accurately measuring TGF-beta1 levels, researchers can gain valuable insights into disease pathology and potentially identify novel therapeutic targets.Overall, the Mouse TGF-beta1 CLIA Kit is an essential tool for studying the role of TGF-beta1 in health and disease, offering researchers a reliable and efficient method for quantifying TGF-beta1 levels in mouse samples.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TGF-β1. You can calculate the concentration of Mouse TGF-β1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
87-101
92-108
Average (%)
91
92
98
1:4
Range (%)
94-111
100-115
99-111
Average (%)
101
105
105
1:8
Range (%)
99-115
86-97
98-114
Average (%)
106
91
106
1:16
Range (%)
87-101
88-102
94-108
Average (%)
93
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
98-112
103
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.63
57.1
242.67
23.35
54.55
266.26
Standard deviation
2.93
6.78
17.57
1.99
3.85
19.22
C V (%)
11.43
11.87
7.24
8.52
7.06
7.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TGF-β1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TGF-β1 in samples. No significant cross-reactivity or interference between Mouse TGF-β1 and analogues was observed.
