The Mouse THP (Tamm-Horsfall Glycoprotein) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of THP levels in mouse serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.THP, or Tamm-Horsfall Glycoprotein, is a key glycoprotein involved in the formation of urinary casts and the regulation of kidney function.
It is known to play a crucial role in various renal disorders, making it a valuable biomarker for studying kidney diseases and developing potential therapies.Overall, the Mouse THP CLIA Kit provides researchers with a powerful tool for investigating the role of THP in kidney function and disease, offering valuable insights into potential therapeutic targets and treatment strategies.
Product Name:
Mouse THP (Tamm Horsfall Glycoprotein) CLIA Kit
SKU:
MOES00549
Target:
Mouse THP (Tamm–Horsfall Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse THP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse THP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse THP. You can calculate the concentration of Mouse THP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
92-103
94-108
Average (%)
98
97
102
1:4
Range (%)
100-115
91-104
87-97
Average (%)
107
98
92
1:8
Range (%)
101-113
95-110
97-112
Average (%)
107
102
104
1:16
Range (%)
102-114
92-103
90-104
Average (%)
108
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.12
3.68
13.34
2.05
3.92
12.01
Standard deviation
0.17
0.33
0.87
0.23
0.41
1.01
C V (%)
8.02
8.97
6.52
11.22
10.46
8.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse THP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse THP in samples. No significant cross-reactivity or interference between Mouse THP and analogues was observed.
