The Human THP (Tamm-Horsfall Glycoprotein) ELISA Kit is specifically designed for the quantitative detection of THP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.THP, also known as uromodulin, is a glycoprotein that is primarily produced by the kidneys and plays a crucial role in the formation of kidney stones. Abnormal levels of THP have been associated with various kidney disorders, making it a valuable biomarker for studying these conditions and potentially developing new diagnostic and therapeutic approaches. With its advanced technology and user-friendly format, the Human THP ELISA Kit is a valuable tool for researchers and healthcare professionals seeking to investigate the role of THP in kidney diseases and other related disorders. Get your kit today and unlock new insights into the intricate mechanisms underlying these conditions.
Product Name:
Human THP (Tamm Horsfall Glycoprotein) ELISA Kit
SKU:
HUES02065
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
