Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
The Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is a specialized assay designed to quantitatively measure levels of TIMP-1 in various biological samples. TIMP-1 is a critical regulator of metalloproteinase activity, playing a significant role in the control of tissue remodeling, cell proliferation, and extracellular matrix maintenance. As an essential component in inhibiting matrix degradation, TIMP-1 is involved in numerous physiological and pathological processes, including wound healing, cancer invasion, and tissue repair. Accurate measurement of TIMP-1 levels is crucial for elucidating its role in tissue homeostasis, disease progression, and potential therapeutic interventions.
Understanding the expression levels and activity of TIMP-1 can provide valuable insights into the pathophysiology of various conditions, particularly those involving tissue remodeling and extracellular matrix dynamics. The Mouse TIMP-1 ELISA Kit from Assay Genie offers high sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research studies investigating the biological functions of TIMP-1.
Product Name:
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
SKU:
AEES00260
Target:
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TIMP-1. You can calculate the concentration of Mouse TIMP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
93-107
91-107
Average (%)
104
100
97
1:4
Range (%)
99-111
92-102
92-105
Average (%)
105
97
100
1:8
Range (%)
99-117
94-105
93-108
Average (%)
106
99
99
1:16
Range (%)
97-113
90-102
91-104
Average (%)
105
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
248.17
730.69
2077.72
233.39
783.18
2236.48
Standard deviation
12.56
43.33
100.35
15.47
41.67
108.47
C V (%)
5.06
5.93
4.83
6.63
5.32
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TIMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TIMP-1 in samples. No significant cross-reactivity or interference between Mouse TIMP-1 and analogues was observed.
