The Mouse TNF-Beta (Tumor Necrosis Factor Beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-Beta levels in mouse serum, plasma, and cell culture supernatants. This kit delivers reliable and reproducible results, making it ideal for a variety of research applications.TNF-Beta is a key cytokine involved in regulating inflammation, immune response, and cell death. Dysregulation of TNF-Beta has been implicated in various inflammatory and autoimmune diseases, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
With its high sensitivity and specificity, the Mouse TNF-Beta CLIA Kit from AssayGenie is the perfect tool for researchers looking to investigate the role of TNF-Beta in disease pathogenesis and explore new treatment strategies. Order yours today and unlock new possibilities in your research endeavors.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNF-β. You can calculate the concentration of Mouse TNF-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
99-112
102-117
Average (%)
101
107
109
1:4
Range (%)
88-104
90-102
89-105
Average (%)
95
97
96
1:8
Range (%)
94-109
88-101
88-104
Average (%)
102
94
95
1:16
Range (%)
87-103
87-100
88-104
Average (%)
94
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.08
64.06
246.14
23.56
62.47
225.3
Standard deviation
2.13
6.48
18.49
2.18
6.58
25.64
C V (%)
9.23
10.12
7.51
9.25
10.53
11.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNF-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNF-β in samples. No significant cross-reactivity or interference between Mouse TNF-β and analogues was observed.
