The Mouse TSLP (Thymic Stromal Lymphopoietin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TSLP levels in mouse serum, plasma, and cell culture supernatants. This kit is perfect for research applications as it provides reliable and reproducible results.TSLP is a key regulatory cytokine involved in the immune response, playing a critical role in conditions such as allergic diseases, asthma, and inflammatory disorders.
By measuring TSLP levels, researchers can better understand the mechanisms underlying these conditions and potentially develop targeted therapies.Overall, the Mouse TSLP CLIA Kit is a valuable tool for studying immune responses and inflammatory diseases in mouse models, providing researchers with essential data to advance their research efforts.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSLP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSLP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TSLP. You can calculate the concentration of Mouse TSLP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
88-101
94-108
Average (%)
92
95
99
1:4
Range (%)
87-99
88-100
95-109
Average (%)
93
95
103
1:8
Range (%)
93-108
101-115
95-111
Average (%)
100
108
102
1:16
Range (%)
88-100
101-116
94-111
Average (%)
94
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-119
108
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.85
69.95
209.0
21.98
65.7
229.43
Standard deviation
2.93
5.31
13.65
1.79
4.85
17.99
C V (%)
12.29
7.59
6.53
8.14
7.38
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TSLP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TSLP in samples. No significant cross-reactivity or interference between Mouse TSLP and analogues was observed.
