The Mouse TXB2 (Thromboxane B2) ELISA Kit is a powerful tool for quantifying thromboxane B2 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and accurate results for a variety of research applications.Thromboxane B2 is a key metabolite of thromboxane A2, a potent mediator of platelet aggregation and vasoconstriction. High levels of thromboxane B2 have been associated with cardiovascular diseases, inflammation, and other pathological conditions.
By measuring thromboxane B2 levels, researchers can gain valuable insights into these processes and potentially identify new therapeutic targets.Whether studying the mechanisms of thromboxane signaling or investigating the role of thromboxane in disease pathology, the Mouse TXB2 (Thromboxane B2) ELISA Kit is an indispensable tool for researchers seeking to advance our understanding of these complex biological processes.
Product Name:
Mouse TXB2 (Thromboxane B2) ELISA Kit
SKU:
MOES01550
Target:
Mouse TXB2 (Thromboxane B2)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXB2. During the reaction, TXB2 in the sample or standard competes with a fixed amount of TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXB2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
99-112
87-103
Average (%)
97
105
95
1:4
Range (%)
83-98
88-100
96-112
Average (%)
90
94
103
1:8
Range (%)
88-103
92-104
99-111
Average (%)
94
98
105
1:16
Range (%)
90-104
90-107
94-112
Average (%)
97
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.2
87.0
453.5
50.0
80.3
446.1
Standard deviation
3.3
5.0
19.0
2.7
3.8
14.3
C V (%)
6.99
5.75
4.19
5.4
4.73
3.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of TXB2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes TXB2 in samples. No significant cross-reactivity or interference between TXB2 and analogues was observed.
