The Mouse UB (Ubiquitin) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of ubiquitin levels in mouse serum, plasma, and cell lysates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Ubiquitin is a small protein that plays a critical role in regulating various cellular processes, including protein degradation, DNA repair, and cell cycle progression. Dysregulation of ubiquitin signaling has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
The Mouse UB CLIA Kit allows researchers to study and monitor ubiquitin levels in mouse models, providing valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Mouse UB (Ubiquitin) CLIA Kit offers researchers a powerful tool for investigating the role of ubiquitin in health and disease, with its high sensitivity and specificity ensuring accurate and reliable results.
Product Name:
Mouse Ub (Ubiquitin) CLIA Kit
SKU:
MOES00587
Target:
Mouse Ub (Ubiquitin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Ub. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Ub and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Ub, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Ub. You can calculate the concentration of Mouse Ub in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
98-110
93-108
Average (%)
97
105
99
1:4
Range (%)
97-111
91-105
100-115
Average (%)
105
97
108
1:8
Range (%)
100-113
94-106
98-111
Average (%)
108
100
103
1:16
Range (%)
94-106
96-112
86-97
Average (%)
100
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.25
89.54
327.76
42.11
91.01
344.23
Standard deviation
5.52
9.36
26.68
3.95
7.39
23.17
C V (%)
12.47
10.45
8.14
9.38
8.12
6.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Ub concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Ub in samples. No significant cross-reactivity or interference between Mouse Ub and analogues was observed.
