Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) CLIA Kit
The Mouse UCP-1 (Uncoupling Protein 1) Mitochondrial CLIA Kit [MOES00400] is a highly reliable and sensitive assay kit designed for the precise measurement of UCP-1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and reproducibility, making it an ideal choice for a wide range of research applications.UCP-1, also known as Uncoupling Protein 1, is a key mitochondrial protein involved in thermogenesis and energy expenditure.
It plays a crucial role in the regulation of metabolism and body temperature, making it a valuable biomarker for studying obesity, diabetes, and metabolic disorders. The Mouse UCP-1 Mitochondrial CLIA Kit is an essential tool for researchers looking to explore the physiological functions of UCP-1 and its implications in various metabolic conditions.
Product Name:
Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) CLIA Kit
SKU:
MOES00400
Target:
Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse UCP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse UCP-1. You can calculate the concentration of Mouse UCP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-119
91-108
99-117
Average (%)
109
98
107
1:4
Range (%)
98-109
102-119
98-110
Average (%)
104
108
103
1:8
Range (%)
85-98
98-117
94-108
Average (%)
91
107
99
1:16
Range (%)
102-115
85-101
95-112
Average (%)
108
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
108
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.08
198.0
694.51
93.34
194.03
644.6
Standard deviation
9.08
23.54
48.96
11.12
20.33
43.25
C V (%)
9.26
11.89
7.05
11.91
10.48
6.71
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse UCP-1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Mouse UCP-1 in samples. No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed.
