Mouse VEGF-B (Vascular Endothelial cell Growth Factor B) CLIA Kit
The Mouse VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit is a reliable and accurate tool for measuring levels of VEGF-B in mouse serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides consistent and trustworthy results, making it an excellent choice for various research purposes.VEGF-B is a key player in angiogenesis, stimulating the growth of blood vessels and influencing cell growth.
Its role in diseases like cancer, cardiovascular disorders, and neurodegenerative conditions makes it a valuable biomarker for understanding these illnesses and exploring potential treatment options.Overall, the Mouse VEGF-B CLIA Kit from Assay Genie offers researchers a powerful tool for studying angiogenesis and its impact on various health conditions, providing valuable insights and potential strategies for therapeutic interventions.
Product Name:
Mouse VEGF-B (Vascular Endothelial cell Growth Factor B) CLIA Kit
SKU:
MOES00589
Target:
Mouse VEGF-B (Vascular Endothelial cell Growth Factor B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGF-B. You can calculate the concentration of Mouse VEGF-B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
94-108
100-113
Average (%)
99
102
107
1:4
Range (%)
100-114
93-106
104-119
Average (%)
108
98
110
1:8
Range (%)
98-112
101-113
99-111
Average (%)
105
106
105
1:16
Range (%)
89-103
90-104
92-103
Average (%)
95
98
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.65
44.93
195.31
26.02
48.09
186.17
Standard deviation
2.95
4.45
16.19
2.22
5.33
17.05
C V (%)
11.97
9.9
8.29
8.53
11.08
9.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VEGF-B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGF-B in samples. No significant cross-reactivity or interference between Mouse VEGF-B and analogues was observed.
