The Mouse VEGF-D (Vascular Endothelial Cell Growth Factor-D) CLIA Kit is a powerful tool for the precise measurement of VEGF-D levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research purposes.VEGF-D, also known as vascular endothelial growth factor D, plays a vital role in promoting the growth and maintenance of blood vessels, contributing to various physiological and pathological processes.
Its involvement in conditions such as cancer, lymphangiogenesis, and inflammation highlights its importance as a biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse VEGF-D CLIA Kit from Assay Genie offers researchers a reliable and efficient solution for investigating the role of VEGF-D in different biological contexts, ultimately advancing our understanding of angiogenesis and related pathways.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGF-D. You can calculate the concentration of Mouse VEGF-D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
102-119
103-118
Average (%)
99
109
109
1:4
Range (%)
102-117
101-117
88-100
Average (%)
108
108
95
1:8
Range (%)
92-104
98-111
91-105
Average (%)
97
103
98
1:16
Range (%)
94-106
97-111
99-113
Average (%)
101
102
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.57
70.7
336.35
36.7
67.99
316.3
Standard deviation
3.55
6.82
26.54
3.77
6.54
33.72
C V (%)
9.71
9.65
7.89
10.27
9.62
10.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VEGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGF-D in samples. No significant cross-reactivity or interference between Mouse VEGF-D and analogues was observed.
