Mouse Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
The Mouse Wingless-Type MMTV Integration Site Family Member 10B (Wnt10b) ELISA Kit is a powerful tool designed for the accurate quantification of Wnt10b levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers robust and reproducible results, making it an invaluable asset for a wide range of research applications.Wnt10b is a key member of the Wnt signaling pathway, regulating various cellular processes such as proliferation, differentiation, and migration. Dysregulation of Wnt10b has been implicated in multiple diseases, including cancer, metabolic disorders, and developmental abnormalities, underscoring its importance as a potential therapeutic target.
By utilizing the Mouse Wnt10b ELISA Kit, researchers can gain valuable insights into the role of Wnt10b in health and disease, paving the way for the development of novel therapeutic strategies and personalized treatment approaches. Trust in this kit to provide accurate and reliable results that will drive impactful discoveries in your research endeavors.
Product Name:
Mouse Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
Product Code:
MODL01397
Size:
96 Assays
Target:
WNT10B
Synonyms:
WNT-12, SHFM6
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
