Description
Monkeypox Virus (MPXV) A33R Protein IgA ELISA Kit
The Monkeypox Virus A33R Protein Human IgA ELISA Kit is specifically designed for the accurate detection of human IgA levels in serum, plasma, and cell culture supernatants in the context of Monkeypox virus infection. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research purposes.The A33R protein is a key component of the Monkeypox virus, playing a crucial role in the virus's pathogenicity and interaction with the host immune system.
By measuring human IgA levels in response to the A33R protein, researchers can better understand the immune response to Monkeypox virus infection and develop potential therapies or vaccines.Overall, the Monkeypox Virus A33R Protein Human IgA ELISA Kit is a valuable tool for studying Monkeypox virus pathogenesis and immune response, providing researchers with the necessary data to advance our understanding of this infectious disease.
| Product Name: | Monkeypox Virus (MPXV) A33R Protein IgA ELISA Kit |
| Product Code: | MKRS0008 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
