Description
Monkeypox Virus (MPXV) A35R Protein IgA ELISA Kit
The Monkeypox Virus A35R Protein Human IgA ELISA Kit is a specialized diagnostic tool designed for the accurate detection of IgA antibodies specific to the A35R protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying Monkeypox virus infection and immunity. The A35R protein is a key antigen involved in the immune response to Monkeypox virus infection, making this kit an essential tool for understanding the host immune response to this pathogen.
By detecting IgA antibodies against the A35R protein, researchers can gain valuable insights into the immune status of individuals exposed to Monkeypox virus and track the progression of infection.This Monkeypox Virus A35R Protein Human IgA ELISA Kit is ideal for a wide range of research applications, including epidemiological studies, vaccine development, and therapeutic research. With its high level of accuracy and precision, this kit is a valuable asset for researchers working in the field of infectious diseases and virology.
| Product Name: | Monkeypox Virus (MPXV) A35R Protein IgA ELISA Kit |
| Product Code: | MKRS0011 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
