Description
Monkeypox Virus (MPXV) C19L Protein IgG ELISA Kit
The Monkeypox Virus C19L Protein Human IgG ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of Monkeypox Virus C19L protein-specific human IgG antibodies in serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it a valuable tool for research and diagnostic applications related to Monkeypox virus infection.The C19L protein is a key component of the Monkeypox virus, which belongs to the Orthopoxvirus genus and is a close relative of the variola virus that causes smallpox.
Detection of specific IgG antibodies against the C19L protein can help in understanding the immune response to Monkeypox virus infection and in developing effective diagnostic and therapeutic strategies.By using the Monkeypox Virus C19L Protein Human IgG ELISA Kit, researchers and healthcare professionals can gain valuable insights into the immune response against Monkeypox virus and contribute to the development of vaccines and treatments for this emerging infectious disease.
| Product Name: | Monkeypox Virus (MPXV) C19L Protein IgG ELISA Kit |
| Product Code: | MKRS0018 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
