Description
Monkeypox Virus (MPXV) H3L Protein IgA ELISA Kit
The Monkeypox Virus H3L Protein Human IgA ELISA Kit is a specialized kit designed for the precise detection of IgA antibodies against the H3L protein of the Monkeypox virus in human serum samples. With its high sensitivity and specificity, this kit ensures accurate and reproducible results for research purposes.The H3L protein is a key component of the Monkeypox virus, playing a crucial role in the virus's replication and pathogenesis. By measuring IgA antibodies against this protein, researchers can gain valuable insights into immune responses against Monkeypox virus infection and potentially develop strategies for diagnosis and treatment.
Ideal for studying infectious diseases and immune responses, the Monkeypox Virus H3L Protein Human IgA ELISA Kit is a valuable tool for researchers looking to advance their understanding of Monkeypox virus and develop novel approaches for combating this infectious agent.
| Product Name: | Monkeypox Virus (MPXV) H3L Protein IgA ELISA Kit |
| Product Code: | MKRS0026 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
