Description
Monkeypox Virus (MPXV) I1L Protein IgA ELISA Kit
The Monkeypox Virus i1L Protein Human IgA ELISA Kit is a cutting-edge tool designed for the precise detection of IgA levels specific to the Monkeypox virus i1L protein in human samples including serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring accurate and reliable results for a wide range of research applications.The Monkeypox virus i1L protein is a key component of the Monkeypox virus, influencing the pathogenesis and immune response to this viral infection. By detecting IgA levels specific to this protein, researchers can gain insight into the immune response to Monkeypox virus infection and potentially develop strategies for preventing and treating this disease.
With its high-quality components and user-friendly protocol, the Monkeypox Virus i1L Protein Human IgA ELISA Kit is a valuable tool for researchers studying Monkeypox virus infection, vaccine development, and immune response mechanisms. With this kit, researchers can advance their understanding of Monkeypox virus pathogenesis and potentially contribute to the development of effective treatments and preventive measures for this infectious disease.
| Product Name: | Monkeypox Virus (MPXV) I1L Protein IgA ELISA Kit |
| Product Code: | MKRS0029 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
