Description
Monkeypox Virus (MPXV) I1L Protein IgG ELISA Kit
The Monkeypox Virus I1L Protein Human IgG ELISA Kit is a powerful tool for the precise measurement of human IgG antibodies targeting the I1L protein of the Monkeypox virus. This kit is designed for use with human serum, plasma, and cell culture supernatants, providing researchers with a reliable and accurate method for studying immune responses to Monkeypox virus infection.The Monkeypox virus is a zoonotic virus that causes a disease similar to smallpox in humans. The I1L protein is an important target for the immune system's response to Monkeypox virus infection, making it a valuable marker for monitoring immune responses and potential vaccine development efforts.
With high sensitivity and specificity, this ELISA kit delivers consistent and reproducible results, making it suitable for a variety of research applications. By accurately quantifying IgG antibodies to the Monkeypox virus I1L protein, researchers can gain valuable insights into the immune response to this infectious pathogen and develop strategies for combating Monkeypox virus infection.
| Product Name: | Monkeypox Virus (MPXV) I1L Protein IgG ELISA Kit |
| Product Code: | MKRS0030 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
