Description
Monkeypox Virus (MPXV) M1R Protein IgA ELISA Kit
The Monkeypox Virus M1R Protein Human IgA ELISA Kit is a highly accurate and reliable tool for the detection of IgA antibodies against the Monkeypox Virus M1R protein in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, allowing for precise and reproducible results across a variety of research applications.The Monkeypox Virus M1R protein is a key component in the immune response to Monkeypox Virus infection, making it a valuable biomarker for studying and understanding the pathogenesis of this virus.
By detecting IgA antibodies against this protein, researchers can gain insights into the immune response and potential therapeutic strategies for combating Monkeypox Virus.Overall, the Monkeypox Virus M1R Protein Human IgA ELISA Kit is an essential tool for researchers studying Monkeypox Virus infection and developing strategies for prevention and treatment. With its high level of accuracy and reliability, this kit is a valuable asset in the fight against Monkeypox Virus.
| Product Name: | Monkeypox Virus (MPXV) M1R Protein IgA ELISA Kit |
| Product Code: | MKRS0035 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
