The MRE11 Antibody (CAB14026) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when used in Western blot applications, enabling accurate detection and analysis of MRE11A in various cell types.MRE11A is a crucial component of the MRN complex, essential for DNA double-strand break repair and the maintenance of genome integrity. Dysregulation of MRE11A has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
MRE11 Antibody
SKU:
CAB14026
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATLD, HNGS1, MRE11A, MRE11B, MRE11
Positive Sample:
HeLa, Jurkat, C6
Cellular Localization:
Nucleus.
Calculated MW:
81kDa
Observed MW:
81kDa
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
4361
RRID
AB_2760881
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRE11 Rabbit pAb (CAB14026) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg MRE11 antibody (CAB14026). Western blot was performed from the immunoprecipitate using MRE11 antibody (CAB14026) at a dilution of 1:1000.
