The MRPL16 Polyclonal Antibody (PAC041722) is a valuable tool for researchers studying mitochondrial ribosomal protein L16 (MRPL16) and its role in cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the MRPL16 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential component in studies related to mitochondrial function and protein synthesis.MRPL16 is a crucial component of the mitochondrial ribosome, playing a vital role in the translation of proteins within the mitochondria.
Understanding the function and regulation of MRPL16 is essential for unraveling the complexities of mitochondrial biology and its implications in various diseases, including metabolic disorders and neurodegenerative conditions. The MRPL16 Polyclonal Antibody provides researchers with a reliable tool to explore the function and regulation of MRPL16 in both normal and pathological cellular processes, paving the way for new insights and potential therapeutic interventions.
Immunohistochemistry of paraffin-embedded human colon cancer using PACO41722 at dilution of 1:100.
Immunofluorescence staining of HepG2 cells with PACO41722 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Component of the large subunit of mitochondrial ribosome.
Synonyms:
39S ribosomal protein L16, mitochondrial (L16mt) (MRP-L16) (Mitochondrial large ribosomal subunit protein uL16m), MRPL16
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
