The MRPS31 Antibody (CAB12923) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the MRPS31 protein, allowing for accurate detection and analysis in various cell types.MRPS31 is essential for maintaining mitochondrial function and has been linked to cellular processes such as oxidative phosphorylation and apoptosis. Dysregulation of MRPS31 has been associated with diseases such as cancer and neurodegenerative disorders, making it a promising target for therapeutic development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
MRPS31 Antibody
SKU:
CAB12923
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
S31mt, IMOGN38, MRP-S31, MRPS31
Positive Sample:
Jurkat, HeLa, HepG2, MCF7, A-431
Cellular Localization:
Mitochondrion.
Calculated MW:
45kDa
Observed MW:
37kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13.
Purification Method
Affinity purification
Gene ID
10240
RRID
AB_2759769
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS31 Rabbit pAb (CAB12923) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Confocal immunofluorescence analysis of U2OS cells using MRPS31 Rabbit pAb (CAB12923) at dilution of 1:200. Blue: DAPI for nuclear staining.
