Description
Mouse HGF ELISA Kit (High Sensitivity)
**** The Mouse HGF (Hepatocyte Growth Factor) ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously designed for the precise quantitative determination of Hepatocyte Growth Factor (HGF) levels in various biological samples collected from mouse models. HGF, a critical signaling molecule, serves as a key regulator of cellular growth, proliferation, and tissue repair processes, making it a pivotal factor in understanding tissue regeneration, organ development, and pathophysiological conditions in mouse studies. This high-sensitivity ELISA kit empowers researchers to explore the intricate functions of HGF with exceptional sensitivity and accuracy. Through the precise measurement of HGF levels, researchers can unravel the multifaceted roles of this growth factor in cellular dynamics, tissue remodeling, and disease progression in mouse experimental models.
The heightened sensitivity of this kit enables detailed investigations into the diverse functions and signaling pathways governed by HGF in mouse biology. The Mouse HGF ELISA Kit - High Sensitivity by Assay Genie guarantees exceptional sensitivity and specificity, ensuring dependable and reproducible results. Crafted under rigorous quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal solution for researchers aiming to decipher the roles and mechanisms of HGF in mouse-related research endeavors.
| Product Name: | Mouse HGF ELISA Kit (High Sensitivity) |
| Product Code: | AEES00602 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 1.56-100 ng/mL |
| Sensitivity: | 0.94 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse HGF concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HGF. You can calculate the concentration of Mouse HGF in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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