Description
Mouse ICAM-1/CD54 ELISA Kit (High Sensitivity)
**** The Mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted for the precise assessment of Intercellular Adhesion Molecule 1 (ICAM-1 or CD54) levels in various mouse biological samples. ICAM-1 is a crucial adhesion molecule involved in immune responses, inflammation, and cellular interactions. By quantifying ICAM-1 levels with high sensitivity, researchers can elucidate its role in cell adhesion, immune cell recruitment, and inflammatory processes, providing valuable insights into disease progression and potential therapeutic interventions. This high-sensitivity ELISA kit empowers researchers to explore the intricate mechanisms underlying ICAM-1 signaling, paving the way for a deeper understanding of its impact on immune responses and inflammatory conditions.
By accurately measuring ICAM-1 levels in mouse samples, researchers can unravel the complexities of immune cell interactions, vascular integrity, and inflammatory cascades, leading to novel discoveries in immunology and disease pathogenesis. The Mouse ICAM-1/CD54 ELISA Kit - High Sensitivity, developed by Assay Genie, ensures exceptional precision, sensitivity, and reproducibility, enabling reliable and high-quality results for advanced research applications. With stringent quality control standards and optimized performance, this kit provides robust and user-friendly protocols, making it an ideal choice for in-depth investigations into ICAM-1 biology and its implications in mouse models.
| Product Name: | Mouse ICAM-1/CD54 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00607 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse ICAM-1/CD54 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ICAM-1/CD54. You can calculate the concentration of Mouse ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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