Description
Mouse IFN-gamma Receptor 1 (IFNGR1) ELISA Kit (High Sensitivity)
**** The Mouse IFN-? R1 (Interferon Gamma Receptor 1) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted for the precise and quantitative measurement of IFN-? R1 levels in various biological samples from mouse models. IFN-? R1 is a key receptor involved in mediating the effects of interferon-gamma, a pivotal cytokine with multifaceted roles in immune responses, inflammation, and anti-pathogen defense.
By offering high sensitivity and specificity, this ELISA kit empowers researchers to delve into the intricate interactions of IFN-? R1 and its downstream signaling pathways, shedding light on its role in immune regulation, host defense mechanisms, and potential implications across different disease contexts. Accurate quantification of IFN-? R1 levels using this specialized kit serves as a crucial stepping stone in unraveling the complexity of immune responses and cytokine signaling cascades. Manufactured with stringent quality control measures by Assay Genie, the Mouse IFN-? R1 ELISA Kit - High Sensitivity ensures robust performance, reliable and reproducible results, and ease of use. Its exceptional sensitivity makes it an ideal choice for researchers seeking to explore the nuances of IFN-? R1 biology and its impact on immune modulation, inflammation, and disease pathogenesis in mouse models.
| Product Name: | Mouse IFN-gamma Receptor 1 (IFNGR1) ELISA Kit (High Sensitivity) |
| Product Code: | AEES00610 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.09 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse IFN-? R1 in samples. No significant cross-reactivity or interference between Mouse IFN-? R1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-? R1 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-? R1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-? R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-? R1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-? R1. You can calculate the concentration of Mouse IFN-? R1 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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