Description
Mouse IGF-1 ELISA Kit (High Sensitivity)
**** The Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit - High Sensitivity is an advanced assay meticulously crafted to quantitatively detect IGF-1 levels in various biological samples from mouse models. IGF-1, a vital growth factor, plays a pivotal role in regulating cell proliferation, growth, and tissue repair, making it a key molecule for investigating growth-related mechanisms, aging processes, and disease conditions in mouse studies. This high-sensitivity ELISA kit offers researchers a precise tool to explore IGF-1 dynamics with exceptional accuracy and sensitivity, enabling detailed investigations into its role in modulating cell growth, metabolic functions, and tissue homeostasis with unparalleled precision.
The enhanced sensitivity of this kit empowers researchers to delve into the intricate signaling pathways and regulatory functions governed by IGF-1 in mouse models with utmost reliability. The Mouse IGF-1 ELISA Kit - High Sensitivity by Assay Genie provides exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly operation, making it the preferred choice for researchers aiming to study the intricate mechanisms and biological functions of Insulin-like Growth Factor 1 in mouse models.
| Product Name: | Mouse IGF-1 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00600 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 1.25-80 ng/mL |
| Sensitivity: | 0.75 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IGF-1 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGF-1. You can calculate the concentration of Mouse IGF-1 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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