Description
Mouse IL-4 ELISA Kit (High Sensitivity)
**** The Mouse IL-4 ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously developed to enable the quantitative measurement of Interleukin-4 (IL-4) levels across various biological samples obtained from mouse models. IL-4, a crucial cytokine recognized for its diverse roles in modulating immune responses, cellular differentiation, and allergic reactions, plays a pivotal function in orchestrating the body's immune defenses and maintaining immune homeostasis.
With exceptional sensitivity, this specialized ELISA kit empowers researchers to delve into the complex intricacies of IL-4-mediated immune responses, inflammatory signaling pathways, and immune cell regulation within mouse models. Providing high sensitivity and precision in detection, this high-sensitivity kit serves as an invaluable tool for investigating the roles of IL-4 in modulating immune function and inflammatory processes. Prioritizing quality and performance, the Mouse IL-4 ELISA Kit - High Sensitivity from Assay Genie ensures the delivery of accurate and reproducible results, facilitating in-depth exploration of IL-4's involvement in immune modulation and disease pathologies. Designed for ease of use and robust performance, this kit is an exceptional choice for researchers conducting studies in immunology, allergy research, and inflammatory diseases utilizing mouse models.
| Product Name: | Mouse IL-4 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00595 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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