Description
Mouse RANTES/CCL5 ELISA Kit (High Sensitivity)
The Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit - High Sensitivity is a specialized research tool designed for the accurate quantitative analysis of RANTES levels in various biological samples from mouse models. RANTES, a potent chemokine, plays a critical role in regulating immune cell migration, inflammation, and immune responses. This high-sensitivity ELISA kit empowers researchers to precisely measure RANTES concentration, providing valuable insights into its involvement in inflammatory processes, immune modulation, and disease pathogenesis within the mouse model context.
Accurate quantification of RANTES is essential for advancing the understanding of its functions in immune regulation and inflammatory diseases. Manufactured to meet rigorous quality control standards, the Mouse RANTES ELISA Kit - High Sensitivity offered by Assay Genie ensures dependable and reproducible results, making it an ideal choice for researchers seeking robust performance and ease of use in their investigative studies.
| Product Name: | Mouse RANTES/CCL5 ELISA Kit (High Sensitivity) |
| Product Code: | AEES00614 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse RANTES concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RANTES. You can calculate the concentration of Mouse RANTES in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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