Description
Mouse P-Selectin/CD62P ELISA Kit (High Sensitivity)
The Mouse SELP (P-Selectin) ELISA Kit - High Sensitivity is a state-of-the-art assay specifically designed to quantitatively measure P-Selectin levels in various biological samples collected from mouse models. P-Selectin, a member of the selectin family, serves as a crucial adhesion molecule involved in the inflammatory response and leukocyte recruitment to sites of inflammation and injury. With high sensitivity, this ELISA kit enables precise quantification of P-Selectin, offering valuable insights into its role in mediating inflammatory processes, endothelial dysfunction, and vascular homeostasis in murine models.
Accurate measurement of P-Selectin levels is essential for elucidating its impact on the immune response and inflammatory cascades. Delivering reliable and reproducible results, the Mouse SELP ELISA Kit - High Sensitivity by Assay Genie is manufactured under strict quality control standards, ensuring robust performance and ease of use for researchers investigating the function and significance of P-Selectin in inflammatory conditions and related diseases.
| Product Name: | Mouse P-Selectin/CD62P ELISA Kit (High Sensitivity) |
| Product Code: | AEES00616 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.09 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse SELP concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. You can calculate the concentration of Mouse SELP in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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