Description
Mouse VEGF-A ELISA Kit (High Sensitivity)
**** The Mouse VEGF-A (Vascular Endothelial Growth Factor A) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously developed for the precise and quantitative measurement of VEGF-A levels in various biological samples from mouse models.
VEGF-A, a vital pro-angiogenic factor, plays a crucial role in the regulation of blood vessel growth and endothelial cell function, impacting various physiological and pathological processes such as angiogenesis, vascular permeability, and tissue repair. This high-sensitivity ELISA kit offers exceptional accuracy and detection limits, enabling researchers to explore the intricate roles of VEGF-A in vascular development, tumor angiogenesis, and various vascular-related disorders with enhanced precision. With unparalleled sensitivity and specificity, this kit ensures reliable and reproducible results, empowering researchers to delve deep into the mechanisms involving VEGF-A in diverse biological contexts. Manufactured under stringent quality control standards, the Mouse VEGF-A ELISA Kit - High Sensitivity provides robust performance and user-friendly protocols, making it an ideal choice for advanced research in vascular biology, angiogenesis, and vascular-targeted therapies within mouse models.
| Product Name: | Mouse VEGF-A ELISA Kit (High Sensitivity) |
| Product Code: | AEES00592 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 3.21 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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