The MT-ND5 Antibody (CAB17972) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.The MT-ND5 protein is a subunit of mitochondrial complex I, which plays a crucial role in the electron transport chain and ATP production within the mitochondria. Dysregulation of complex I function has been implicated in various human diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MT-ND5 Antibody
SKU:
CAB17972
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MTND5, ND5, MT-ND5
Positive Sample:
HepG2, Mouse heart
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrial Respiratory Chain Complex I.
Calculated MW:
67kDa
Observed MW:
70kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and MELAS syndrome.
Purification Method
Affinity purification
Gene ID
4540
RRID
AB_2861774
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MT-ND5 Rabbit pAb (CAB17972) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using MT-ND5 Rabbit pAb (CAB17972) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MT-ND5 Rabbit pAb (CAB17972) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
