The MT-ND5 Antibody (CAB17972) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and MELAS syndrome.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MT-ND5 Antibody
SKU:
CAB17972
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MTND5, ND5, MT-ND5
Positive Sample:
HepG2, Mouse heart
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrial Respiratory Chain Complex I.
Calculated MW:
67kDa
Observed MW:
70kDa
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and MELAS syndrome.
Purification Method
Affinity purification
Gene ID
4540
RRID
AB_2861774
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MT-ND5 Rabbit pAb (CAB17972) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using MT-ND5 Rabbit pAb (CAB17972) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MT-ND5 Rabbit pAb (CAB17972) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
