The MYH6/alpha-MHC Antibody (CAB12964) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. RRID AB_2759811 Gene ID 4624 Swiss Prot Synonym ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC; MYH6/α-MHC
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MYH6/alpha-MHC Antibody
SKU:
CAB12964
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:5000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. RRID AB_2759811 Gene ID 4624 Swiss Prot Synonym ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC; MYH6/α-MHC
Purification Method:
Affinity purification
Gene ID:
4624
RRID:
AB_2759811
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MYH6/α-MHC Rabbit pAb (CAB12964) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse heart using MYH6/α-MHC Rabbit pAb (CAB12964) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded mouse heart using MYH6/α-MHC Rabbit pAb (CAB12964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of paraffin-embedded rat heart using MYH6/α-MHC Rabbit pAb (CAB12964) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
