The MYH7B Monoclonal Antibody (CAB19246) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, exhibits high specificity and sensitivity for detecting MYH7B in human samples, making it ideal for use in various experimental techniques such as Western blotting and immunofluorescence.MYH7B, a member of the myosin superfamily, is known for its role in striated muscle contraction and its involvement in muscle fiber development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MYH7B Monoclonal Antibody
SKU:
CAB19246
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2406
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MHC14, MYH14, lncMYH7b, MYH7B
Positive Sample:
Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Membrane.
Calculated MW:
226kDa
Observed MW:
226kDa
The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined.
Purification Method
Affinity purification
Gene ID
57644
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using MYH7B Rabbit mAb (CAB19246) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using MYH7B Rabbit mAb (CAB19246) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
