The MYO1B Monoclonal Antibody (CAB21113) is a high-quality antibody developed for reliable detection and analysis of target proteins. This specific monoclonal antibody, generated in mice, exhibits strong reactivity with human MYO1B protein and is suitable for use in Western blot and immunofluorescence applications.MYO1B is known to play a critical role in cell motility, actin cytoskeleton organization, and lipid droplet dynamics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYO1B Monoclonal Antibody
SKU:
CAB21113
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2990
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MMIa, myr1, MYH-1c, MMI-alpha, MYO1B
Positive Sample:
A-431, Mouse lung, Rat lung
Cellular Localization:
Actin Cytoskeleton, Cytoplasm, Early Endosome, Extracellular Exosome, Filopodium, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
132kDa
Observed MW:
132kDa
Enables ATP binding activity; actin filament binding activity; and microfilament motor activity. Involved in actin filament organization and post-Golgi vesicle-mediated transport. Located in several cellular components, including actin filament; endosome; and perinuclear region of cytoplasm. Colocalizes with trans-Golgi network membrane.
Purification Method
Affinity purification
Gene ID
4430
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung, using MYO1B Rabbit mAb (CAB21113) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using MYO1B Rabbit mAb (CAB21113) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
