The N-Cadherin Polyclonal Antibody is a vital tool for researchers studying N-cadherin, a key protein involved in cell-cell adhesion and signaling. This antibody, produced in rabbits, demonstrates high specificity and sensitivity when used with human samples, making it ideal for Western blot applications.N-Cadherin plays a crucial role in various biological processes, including tissue development, neuronal migration, and tumor metastasis. By targeting N-cadherin, researchers can gain insights into these processes and potentially develop new therapeutic strategies for diseases such as cancer.
With the ability to detect and analyze N-cadherin in a variety of cell types, this polyclonal antibody is a valuable asset for studies in developmental biology, neuroscience, and cancer research. Its versatility and reliability make it a valuable addition to any laboratory conducting research into cell adhesion and signaling mechanisms.
Product Name:
N-Cadherin Polyclonal Antibody
SKU:
CAB21308
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 807-906 of human N-Cadherin (NP_001783.2).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
CDHN; NCAD; ACOGS; ADHD8; CD325; ARVD14; CDw325; in
Positive Sample:
HeLa,293T
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
100kDa
Observed MW:
140kDa
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Purification Method:
Affinity purification
Gene ID:
1000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] N-Cadherin Rabbit pAb (CAB21308) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
_WB_01__42794.1701341189.jpg?c=2?imbypass=on "Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (CAB21308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
_WB_01__42794.1701341189.jpg?c=2 "Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (CAB21308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
