Description
N6-methyladenosine/m6A Monoclonal Antibody (CAB19841)
The N6-methyladenosine/m6A Monoclonal Antibody (CAB19841) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for detecting m6A, a common RNA modification that plays a crucial role in regulating gene expression and protein translation.With validated use in various applications such as immunofluorescence and immunohistochemistry, this antibody allows for precise detection and analysis of m6A in different cell types and tissues. Its high reactivity with human samples makes it an ideal choice for studies in cancer, neurobiology, and developmental biology, where m6A has been implicated in various cellular processes.
This antibody is validated for use in IF/ICC, ELISA, Nucleotide Array, DB, meRIP applications and has demonstrated reactivity against Species independent samples.
| Product Name: | N6-methyladenosine/m6A Monoclonal Antibody |
| SKU: | CAB19841 |
| Size: | 20μL, 100μL |
| Reactivity: | Species independent |
| Clone Number: | ARC5003-10 |
| Immunogen: | Chemical compounds corresponding to N6-methyladenosine / m6A. | ||||||||
| Tested Applications: | IF/ICC ELISA Nucleotide Array DB meRIP | ||||||||
| Recommended Dilution: |
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| Synonyms: | N6-methyladenosine, m6A, N6-methyladenosine / m6A |
| Observed MW: | Refertofigures |
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
| Purification Method | Protein A |
| RRID | AB_2862753 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | The m6A rabbit monoclonal antibody (CAB19841) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8 - ATAACTGG-m6A-CCGAATGG Oligomer 9 - ATAACTGGACCGAATGG Oligomer 10 - AAAAAAAAAAAAAAAA-biotin. |
 | Confocal imaging of U-2 OS cells (treated with UV and 6min Brdu) and U-2 OS cells (treated with UV and 0min Brdu) using N6-methyladenosine / m6A Rabbit mAb (CAB19841, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
