The TBK1/NAK Monoclonal Antibody (CAB3458) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.NAK/TBK1, also known as TANK-binding kinase 1, is a serine/threonine protein kinase that is involved in the regulation of antiviral responses and inflammatory signaling pathways. Dysregulation of NAK/TBK1 has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TBK1/NAK Monoclonal Antibody
SKU:
CAB3458
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0778
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NAK, T2K, IIAE8, FTDALS4, TBK1/NAK
Positive Sample:
Hep G2, Mouse lung, Mouse testis, Rat testis, Rat lung, 293T, Raji
Cellular Localization:
Cytoplasm.
Calculated MW:
84kDa
Observed MW:
84kDa
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.
Purification Method
Affinity purification
Gene ID
29110
RRID
AB_2863061
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of lysates from wild type (WT) and TBK1/NAK knockout (KO) 293T cells using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of MCF7 cells using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using [KO Validated] TBK1/NAK Rabbit mAb (CAB3458,at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
