The Alpha SNAP Monoclonal Antibody (CAB19227) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, has high reactivity with human samples and has been validated for use in various applications, including Western blot and immunofluorescence.Alpha SNAP plays a crucial role in the regulation of vesicle fusion and neurotransmitter release, making it a significant target for studies in neuroscience and cell biology. By binding to Alpha SNAP, this antibody enables the detection and analysis of the protein in different cell types, providing valuable insights into its function and regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Alpha SNAP Monoclonal Antibody
SKU:
CAB19227
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2382
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SNAPA, Alpha SNAP
Positive Sample:
HepG2, A-549, Mouse brain, Rat brain
Cellular Localization:
Membrane, Peripheral Membrane Protein.
Calculated MW:
33kDa
Observed MW:
33kDa
This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.
Purification Method
Affinity purification
Gene ID
8775
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Alpha SNAP Rabbit mAb (CAB19227) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
