The NCK1 Antibody (CAB0999) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the NCK1 protein, this antibody allows for the detection and analysis of NCK1 expression in a variety of cell types, making it an essential component of studies in cell biology and cancer research.NCK1 is a critical mediator of intracellular signaling pathways that regulate cell proliferation and migration. Its involvement in these processes makes it an important target for investigation in diseases such as cancer, where aberrant signaling can contribute to tumor growth and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
NCK1 Antibody
SKU:
CAB0999
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NCK, nck-1, NCKalpha, NCK1
Positive Sample:
Mouse brain
Cellular Localization:
Cytoplasm, Endoplasmic Reticulum, Nucleus.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Purification Method
Affinity purification
Gene ID
4690
RRID
AB_2757518
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse brain, using NCK1 Rabbit pAb (CAB0999) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
