The NCS1 Antibody (CAB13586) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through rabbit immunization, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the NCS1 protein, this antibody enables researchers to detect and analyze NCS1 expression in different cell types, making it a versatile choice for neuroscience and neurology studies.NCS1, also known as frequenin, is a key player in calcium signaling pathways within neurons, impacting neurotransmission and neuronal excitability.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NCS1 Antibody
SKU:
CAB13586
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
23413
RRID
AB_2760448
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NCS1 Rabbit pAb (CAB13586) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A549 cells using NCS1 Rabbit pAb (CAB13586). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.