The NDUFAB1 Antibody (CAB14657) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and sensitivity for human samples, making it ideal for use in Western blot applications.NDUFA13 is an essential component of Complex I, which plays a critical role in electron transport and energy production within the mitochondria. Dysregulation of Complex I function has been implicated in various disorders, including neurodegenerative diseases and metabolic conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFAB1 Antibody
SKU:
CAB14657
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACP, ACP1, SDAP, FASN2A, NDUFAB1
Positive Sample:
293T, NCI-H460, Mouse kidney, Mouse heart, Rat kidney, Rat heart
Cellular Localization:
Mitochondrion.
Calculated MW:
17kDa
Observed MW:
12kDa
Predicted to enable acyl binding activity; acyl carrier activity; and fatty acid binding activity. Involved in mitochondrial respiratory chain complex I assembly and protein lipoylation. Located in mitochondrion and nucleoplasm. Part of mitochondrial respiratory chain complex I. Colocalizes with mitochondrial large ribosomal subunit.
Purification Method
Affinity purification
Gene ID
4706
RRID
AB_2761533
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFAB1 Rabbit pAb (CAB14657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using NDUFAB1 Rabbit pAb (CAB14657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
