The Neurofilament M Monoclonal Antibody (CAB19085) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting NEFM in human samples, making it a reliable choice for experiments such as Western blotting and immunohistochemistry.NEFM, also known as neurofilament medium chain, is essential for the functioning of nerve cells and is involved in various neurological disorders such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS).
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Neurofilament M Monoclonal Antibody
SKU:
CAB19085
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0396
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ELVA DAKV EKPE KAKS PVPK SPVE EKGK SPVP KSPV EEKG KSPV PKSP VEEK GKSP VPKS PVEE KGKS PVSK SPVE EKAK SPVP KSPV EEAK SKAE VGKG E
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method
Affinity purification
Gene ID
4741
RRID
AB_2862577
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Neurofilament M Rabbit mAb (CAB19085) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal imaging of SH-SY5Y cells using Neurofilament M Rabbit mAb (CAB19085,dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
